The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer.
- The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer.
- Medzihradszky, Katalin F.; Campbell, Jennifer M.; Baldwin, Michael A.
- S.l. : s.n., n.d
- Analytical chemistry. vol. 2000(72), 3. p. 552-558
- Samenvatting:A new matrix-assisted laser desorption/ionization (MALDI)time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem massspectrometer is described for sequencing peptides, This instrumentcombines the advantages of high sensitivity for peptide analysisassociated with MALDI and comprehensive fragmentation informationprovided by high-energy collision-induced dissociation (CID). Unlike thepostsource decay technique that is widely used with MALDI-TOF instrumentsand typically combines as many as 10 separate spectra of different massregions, this instrument allows complete fragment ion spectra to beobtained in a single acquisition at a fixed reflection voltage. Toachieve optimum resolution and focusing over the whole mass range, it maybe desirable to acquire and combine three separate sections. Differentcombinations of MALDI matrix and collision gas determine the amount ofinternal energy deposited by the MALDI process and the CID process, whichprovide control over the extent and nature of the fragment ions observed.Examples of peptide sequencing are presented that identifysequence-dependent features and demonstrate the value of modifying theionization and collision conditions to optimize the spectral information.