Maleylacetate reductase of Pseudomonas sp. strain B13 : dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds.

Type:
artikel
Titel:
Maleylacetate reductase of Pseudomonas sp. strain B13 : dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds.
Auteur:
Kaschabek, Stefan R.; Reineke, Walter
Taal:
Nederlands
Uitgever:
S.l. : s.n., n.d
Paginering:
6 p.
Nota:
Archives of Microbiology. (1992)158. pp.412-417. 03028933
Plaatsnummer:
Samenvatting:
The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.
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